mouse bmsc line  (iCell Bioscience Inc)

Journal: Biomaterials

Article Title: Genetic and pharmacologic suppression of PPARγ enhances NELL-1-stimulated bone regeneration

doi: 10.1016/j.biomaterials.2022.121609

Figure Lengend Snippet: (5A) BMSCs were isolated from senile osteopenic mice on day 3 and 7 post-injection. (5B) Quantification of Alizarin Red mineralization staining after BMSCs were isolated and cultured in growth medium for 3 days. (5C) M2–10B4 cells Oil Red O lipid accumulation staining after 14 days of adipogenic induction. (5D) Quantification of relative Oil Red O-positive staining area compared to the total cell area. (5E-H) RT-PCR quantification of OCN, RUNX2, PPARγ, and CEBPα from senile osteopenic mouse BMSCs isolated at baseline (day 0), day 3, and day 7 post-injection. Data are presented as mean ± SD. * p <0.05, ** p <0.01.

Article Snippet: Cells from an immortalized mouse bone marrow-derived stem cell (BMSC) cell line, M2–10B4 (M2) (ATCC, Manassas, VA), were used and maintained in Roswell Park Memorial Institute (RPMI) 1640 growth medium (Invitrogen, Carlsbad, CA) with the addition of 10% fetal bovine serum (FBS), 1 mmol/L sodium pyruvate, and 100 U/mL penicillin/streptomycin (Gibco).

Techniques: Isolation, Injection, Staining, Cell Culture, Reverse Transcription Polymerase Chain Reaction

Journal: PLoS ONE

Article Title: GLIPR1 expression is reduced in multiple myeloma but is not a tumour suppressor in mice

doi: 10.1371/journal.pone.0228408

Figure Lengend Snippet: ( A ) The expression of Glipr1 mRNA was assessed by RT-PCR in CD138 + PCs from healthy WT (C57BL/6) mice and KaLwRij mice, as well as the KaLwRij-derived 5TGM1 MM PC line. The products were run on a 2% agarose gel and stained with GelRed. Actb was used as a positive control. NTC = no template control. ( B ) The expression of Glipr1 protein in 5TGM1-Glipr1 (Glipr1) and control 5TGM1-EV (EV) cells was assessed by Western blot. Hsp90 was used as the loading control. The number of 5TGM1-Glipr1 cells in mono-culture ( C ) or co-culture with OP9 bone marrow stromal cells ( D ) was assessed by measuring luciferase activity after 3 days. Cell number is expressed relative to the EV control cells. ( E ) Colony formation by 5TGM1-Glipr1 cells versus 5TGM1-EV cells was assessed in semi-solid methylcellulose-containing medium after 12 days. Colony number is expressed relative to the EV control cells. Graphs depict the mean + SD of n = 3 independent experiments.

Article Snippet: The mouse BM stromal cell (BMSC) line OP9 was obtained from the ATCC and was maintained in Dulbecco's Modified Eagle Medium (DMEM) with 10% FCS.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Derivative Assay, Agarose Gel Electrophoresis, Staining, Positive Control, Control, Western Blot, Co-Culture Assay, Luciferase, Activity Assay